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KMID : 0380020210360030204
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Korean Journal of Biotechnology and Bioengineering
2021 Volume.36 No. 3 p.204 ~ p.208
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Effect of Ectoine on Biological Membranes with Respect to Phase of the Membranes
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Kang Min-Kyeong
Park Jin-Won
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Abstract
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The effect of the ectoine incorporation on the biological membranes was investigated with respect to the phase of the membranes using the fluorescence intensity change. Spherical phospholipid bilayers, vesicles, were prepared with each layer via a double emulsion technique. In the aqueous inside of the vesicles, 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt(ANTS) was encapsulated. As a quencher, p-Xylene-bis(N-pyridinium bromide)(DPX) was included in the buffer where the vesicles were dispersed. The fluorescence scale was calibrated with the fluorescence of ANTS vesicles in p-Xylene-bis(N-pyridinium bromide)(DPX)-included-buffer taken as 100% fluorescence and the mixture of ANTS and DPX in the buffer as 0% fluorescence. Ectoine injection into the vesicle solution led the change in the membrane structure. It was found that the change was related to the phase of each layer the vesicle up on the ratio of ectoine to lipid. These results seem to depend on the stability of the vesicles, due to the osmotic and volumetric effects on the packing disruption of both head-group and tail-group.
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KEYWORD
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ectoine, vesicles, lipid layer phase
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